How does UV-Vis spectrophotometry work?

Here’s a explanation of how UV-Vis spectrophotometry works:

1. A special light, either ultraviolet (UV) or visible (Vis), is shined on the sample.
2. The light passes through a device that selects one color (wavelength) of light at a time.
3. The light then goes through the sample solution in a transparent container called a cuvette.
4. Some of the light is absorbed by the molecules in the sample, and some passes through.
5. A detector measures how much light passes through the sample.
6. The instrument then tells us how much light was absorbed, which helps us figure out the amount and type of substance in the sample.

In simple words: UV-Vis spectrophotometry works by shining light through a sample and measuring how much light the sample absorbs. The more it absorbs, the more of that substance is present.