Spectrophotometry has different types based on the kind of light used and what is being measured. Here are the main ones explained:
1. UV-Visible (UV-Vis) Spectrophotometry
- Uses ultraviolet and visible light.
- Measures how much light a solution absorbs.
- Used to find the concentration of compounds like proteins or DNA.
2. Infrared (IR) Spectrophotometry
- Uses infrared light.
- Measures how molecules absorb heat energy.
- Helps identify chemical bonds and types of molecules.
3. Atomic Absorption Spectrophotometry (AAS)
- Measures how much light is absorbed by atoms.
- Used to detect metals such as iron, calcium, or lead in samples.
4. Flame Photometry
- Measures the light given off by elements when heated in a flame.
- Commonly used to test for metals like sodium, potassium, and calcium.
5. Atomic Emission Spectrophotometry
- Similar to flame photometry but uses plasma or electric arc.
- Measures light from excited atoms to find and measure elements.
6. X-ray Spectrophotometry
- Uses X-rays to study how materials absorb or emit radiation.
- Used to find elements in solid materials like metals or rocks.
In short:
Different types of spectrophotometry use different kinds of light — UV, visible, infrared, or X-rays — to measure or identify substances and find out how much of them are present.