Here’s a explanation of split and splitless injection in GC:
1. Split Injection
- Only a small part of the sample goes into the column.
- The rest is vented out.
- Used for: Samples with high concentration.
- Pros: Sharp peaks, column doesn’t get overloaded.
- Cons: Not good for very tiny amounts.
2. Splitless Injection
- Almost all of the sample goes into the column.
- The split vent is closed during injection.
- Used for: Trace or very dilute samples.
- Pros: Very sensitive.
- Cons: Column can overload if sample is too strong.
In short:
- Split → small amount in → high concentration.
- Splitless → all sample in → low concentration.